How do you sterilize a plastic pipette?

Pipettes should be autoclaved according to the manufacturer’s instructions. To achieve sterility, a holding time of at least 20 minutes at 121°C (252°F) is required.

Can plastic pipette tips be autoclaved?

Is it necessary to disinfect pipette?

Why should pipette tips be autoclaved?

How do you sterilize a PCR tube?

For PCR I expose tubes under UV while for managing RNA I usually put clean tubes in a glass box, cover it with alluminium and autoclave it for 20 minutes and once dried I put them under UV …. ^_^

How do you clean a plastic dropper?

Simply pull and wiggle the pipette a bit to get it free from the cap. As with the guide above: put the glass pipettes and plastic heads in with your bottles soak overnight. When they’re done soaking, you can use a cotton bud and soapy water to clean the inside of the pipette and dropper.

How do you reuse pipette tips?

Load the tips into an existing pipette tip rack by placing a refill insert into an existing pipette tip rack and firmly press down to dispense. These refill inserts come stacked in a cardboard sleeve that unfolds and reseals for efficient storage.

How do you make a pipette without contamination?

Keep the pipette vertical when pipetting in order to prevent liquid from running into the pipette body. Release the push button slowly. To avoid aerosol contamination, use filter tips or use a positive displacement pipette and tips.

How is DNA removed from the surface?

Household bleach (sodium hypochlorite) is effective for removal of DNA from surfaces [2]. Use freshly prepared solution of household bleach (1 % sodium hypochlorite) [3] for 30 minutes of contact time on the surface followed by rinsing with ethanol or water.

Can I autoclave PCR plates?

Biotix discourages autoclaving of robotic pipette tips, PCR tubes and PCR plates due to the warping/ distortion that autoclaving can introduce to these high precision products.

How do you calibrate a volumetric flask?

How do you calibrate a burette?

Touch the tip of the buret to the side of a beaker to remove the drop hanging from the tip. After about a minute, to allow for drainage, make an initial reading of the meniscus, estimating the volume to the nearest 0.01 mL. Record the initial reading. Allow the buret to stand for 5 minutes and recheck the reading.

How do you calibrate a top pan balance?

To calibrate, you simply need to place a calibration weight on the pan and record the result. If it is within predetermined limits then no further action is required. If the result is not within limits then the balance should be adjusted to the value of the calibration weight.

How do you sterilize large glass bottles?

How do you sterilize a pipette pump?

For aqueous solutions, rinse the contaminated parts with distilled water or 70 percent ethanol and air dry at 60°F. For infectious liquids, autoclave the lower section at a temperature of 120°C for 20 minutes and then allow it to return to room temperature before reassembling.

How do you calibrate a glass pipette?

How do you clean a pipette after use?

For organic solvents, allow the substance to evaporate on its own or immerse the part in a detergent and then air dry. For radioactive substances, place the pipette in a solution like Decon and then rinse and air dry. For proteins, rinse the contaminated parts with a detergent, rinse, and air dry.

How do you sterilize a plastic pipette?

Pipettes should be autoclaved according to the manufacturer’s instructions. To achieve sterility, a holding time of at least 20 minutes at 121°C (252°F) is required.

Does Soap destroy DNA?

soap dissolves the cell and nuclear membranes that protect the DNA. The meat tenderizer and salt help control enzymes in the cells and keep the DNA structurally intact.)

Does water destroy DNA?

DNA is vulnerable. It breaks down in sunlight and water, and there are enzymes that naturally destroy it. But long after death, samples would survive in teeth and bones.

How do you sterilize Eppendorf tubes?

I sterelized eppendorf tubes for RNA extraction samples by placing them in beaker covered with Aluminum foil and autoclave for 15 minute at 121°C.